Matcha & Cancer

In research published in the journal Aging, (See below for the reference) a team from the Biomedical Research Centre at the University of Salford, used metabolic phenotyping on cell lines of breast cancer stem cells and found that green tea Matcha “shifted cancer cells towards a quiescent metabolic state” and stopped their spread at a relatively low concentration (0.2 mg/ml).

They also found that the signalling pathways that promote cancer stem cells indicated that Matcha “strongly affected mTOR signals, weakening components of the 40S ribosome. This raised the possibility that Matcha (Green Tea Match or GTM) could be used in place of chemical drugs such as rapamycin”. (Exhibit A)

Molecular Mechanisms

Up until recently,  the anti-cancer molecular mechanism of matcha have been confusing. However, with this study, by using metabolic phenotyping,  it was found that the matcha tea is suppressing oxidative mitochondrial metabolism in cancer cells, which means that it is preventing the cells from ‘re-fuelling’ and therefore they become inactive and die a natural apoptotic death.

“Our results are consistent with the idea that Matcha may have significant therapeutic potential, mediating the metabolic reprogramming of cancer cells.” (Ibid)

There are multiple signaling pathways involved.  MGT-treated cells display an increase in the beta-oxidation pathway, most likely in an attempt to compensate for the decreased mitochondrial respiration and glycolysis. IPA analysis of proteomics data detected alterations in cell cycle regulation after treatment with MGT. The p53 tumour suppressor protein, which is a transcription factor, stabilized and activated in response to a range of cellular stresses including hyper-proliferation. This study also  demonstrated that treatment with MGT up-regulates the enzymes of the oxidative branch of the pentose phosphate pathway. So that’s pretty keen. Furthermore, IPA analysis showed that treatment with MGT affected the IL-8 pathway. IL-8 signaling is involved in angiogenesis, proliferation and increment of the migratory capacity of cancer cells, in particular cancer stem cells.

Discussion

Stem cells are the body’s basic raw materials out of which specialized cells are generated: blood cells, brain cells, and bone marrow for example. Cancer stem cells come from stem cells..  Chemotherapy and drugs can be targeted to attack the tumor cells in a growing variety of ways. The general principle is to persuade them to commit suicide—apoptosis—by interfering with the messages that regulate their interaction with other cells. Howeverr, the traditional pharmacopeia and chemo don’t kill off the stem cells. The tumor shrinks but over time it grows back. (See Blog under cancer stem cell)

The matcha study is the part of the field of molecular biogenetics that is generally called the Stem Cell Theory as to the role of stem cells in cancer growth and the design of CSC therapies is still debated, notwithstanding hard evidence that they are the engines of metastases (See Blog). There is a great deal of debate about their origin, structure and organization, how they grow and stop growing, de-differentiation, clonal evolution and stochastic alternative theories, mutations, response to extra-cellular signals and more.

Over the past years, the anti-cancer stem cell properties of green tea or its components have been investigated. Its effectiveness is reported across several types of tumor. Previous studies have reported that green tea and epigallocatechin gallate (EGCG), one of the main components of green tea, suppresses cancer stem-like cells (CSCs) properties in a variety of cellular models (1-3)  

This study we have before the Court of expertise was aimed to investigate if the inhibitory efficacy of MGT on CSCs is associated with inhibition of cellular metabolism. The results presented  demonstrate beyond any reasonable doubt  that MGT preferentially inhibits CSC proliferative expansion derived from MCF7 breast cancer cells.

Conclusion and the ACR Institute’s Human Clinical Trial Project

The study’s conclusion, in pertinent part, is as follows:

“In summary, we found that the natural compound Matcha green tea mechanistically targets oxidative phosphorylation and therefore CSC propagation. Importantly, we demonstrated that MGT effectively down-regulated oxygen consumption rate (OCR) and extracellular acidification rate (ECAR). Moreover, MGT treatment impaired others cellular metabolic pathways, and several cell signaling pathways such as cell cycle regulation, antioxidant response and inflammation. Altogether, these findings strongly propose MGT as a natural compound that may help to overcome cancer cell resistance to chemotherapy”.

This Matcha study is great news because its therapeutic molecules target the engine of metastases, the cancer stem cells. As the Institute has been saying for decades, both chemo and radiation are instrincally and structurally flawed in that they not only dont destroy these CSCs, they also reinforce them. (See Blog) Hence the cancer reccurence and chemo-resistance.

From the viewpoint of compelling hard evidence, this study has its limits.  It cell lines were experimented within Petri dishes, so we have a long way to go before clinical human trials are financed and published. At the Institute’s French retreat center, we have nonetheless clinical experience that organic matcha appears to help lots with regard to cancer control and reversal. But because the Institute’s lifestyle holistic protocol that we are trying to verify via a human clinical trial involves so many other variables that it is not possible to attribute its success to this or that compound. With this study, we now have additional proof that Matcha tea, globally, with its many tea’s molecules, from EGCG to other phenolic compounds, modulates the growth of recurring stem cancer cells in Petri dishes. This is a good start.

Ch. J. (ACR Institute director)

Reference Notes

1. Chung SS, Vadgama JV. Curcumin and epigallocatechin gallate inhibit the cancer stem cell phenotype via down-regulation of STAT3-NFκB signaling. Anticancer Res. 2015; 35:39–46. [PubMed]
2. Kumazoe M, Takai M, Hiroi S, Takeuchi C, Yamanouchi M, Nojiri T, Onda H, Bae J, Huang Y, Takamatsu K, Yamashita S, Yamada S, Kangawa K, et al. PDE3 inhibitor and EGCG combination treatment suppress cancer stem cell properties in pancreatic ductal adenocarcinoma. Sci Rep. 2017; 7:1917. https://doi.org/10.1038/s41598-017-02162-9 [PubMed]
3. Fujiki H, Sueoka E, Rawangkan A, Suganuma M. Human cancer stem cells are a target for cancer prevention using (-)-epigallocatechin gallate. J Cancer Res Clin Oncol. 2017; 143:2401–12. https://doi.org/10.1007/s00432-017-2515-2 [PubMed]

Exhibit A

“Matcha green tea (MGT) is a natural product that is currently used as a dietary supplement and may have significant anti-cancer properties. However, the molecular mechanism(s) underpinning its potential health benefits remain largely unknown. Here, we used MCF7 cells (an ER(+) human breast cancer cell line) as a model system, to systematically dissect the effects of MGT at the cellular level, via i) metabolic phenotyping and ii) unbiased proteomics analysis. Our results indicate that MGT is indeed sufficient to inhibit the propagation of breast cancer stem cells (CSCs), with an IC-50 of ~0.2 mg/ml, in tissue culture. Interestingly, metabolic phenotyping revealed that treatment with MGT is sufficient to suppress both oxidative mitochondrial metabolism (OXPHOS) and glycolytic flux, shifting cancer cells towards a more quiescent metabolic state. Unbiased label-free proteomics analysis identified the specific mitochondrial proteins and glycolytic enzymes that were down-regulated by MGT treatment. Moreover, to discover the underlying signalling pathways involved in this metabolic shift, we subjected our proteomics data sets to bio-informatics interrogation via Ingenuity Pathway Analysis (IPA) software. Our results indicate that MGT strongly affected mTOR signalling, specifically down-regulating many components of the 40S ribosome. This raises the intriguing possibility that MGT can be used as inhibitor of mTOR, instead of chemical compounds, such as rapamycin. In addition, other key pathways were affected, including the anti-oxidant response, cell cycle regulation, as well as interleukin signalling. Our results are consistent with the idea that MGT may have significant therapeutic potential, by mediating the metabolic reprogramming of cancer cells”. (Source)
Matcha green tea (MGT) inhibits the propagation of cancer stem cells (CSCs) by targeting mitochondrial metabolism, glycolysis and multiple cell signalling pathways is published in Aging and authored by Gloria Bonuccelli, Federica Sotgia and Michael P Lisanti (Source)

 

Selective List of Green Tea and Cancer Research

Recovering from Breast Cancer with Matcha

Determination of catechins in matcha green tea by micellar electrokinetic chromatography

Green tea: prevention and treatment of cancer by nutraceuticals

Green tea and prostate cancer

Green Tea Polyphenol Epigallocatechin 3-Gallate, Contributes to the Degradation of DNMT3A and HDAC3 in HCT 116 Human Colon Cancer Cells

Nutraceuticals as new treatment approaches for oral cancer: II. Green tea extracts and resveratrol

Role of epigallocatechin gallate (EGCG) in the treatment of breast and prostate cancer

Green tea: An effective synergist with anticancer drugs for tertiary cancer prevention

Green tea: An effective synergist with anticancer drugs for tertiary cancer prevention

Evidence that Nigella Sativa  (black seed oil) is clinically superior to many cancer drugs.

Copyright (c) 2015: Advanced Cancer Research Institute, Pr. Joubert and agents. All rights reserved. In terms of “fair use”,  the Institute allows the free use of this posted article provided it is not altered, full attribution is included as well as the Institute’s Post URL link. Furthermore, consistent with “fair use” case law,  small sections from the Institute’s posted article can be used as long as the above mentioned attributions are made and as long as the usage purpose is based on furthering education and science. For any other usage reason, written permission is required.
DISCLAIMER. Nothing in this blog-webstie is to be construed as medical or legal advise, including, but not limited to replies, comments and posts, all of which can not be deemed to constitute either a therapist-patient nor an attorney-client relationship. This website-blog has been designed to be for educational and heuristic purposes. The information posted therein and available to the public is not intended nor implied to be a substitute for competent professional medical or legal advice.  Always seek the advice of a qualified physician or health-care provider prior to making decisions about diagnosis and treatment. Neither ACR Institute’s founder, not any of its agents  is liable for possible damage incurred as a direct or indirect consequence of using the website-blog’s contents. Nor are liable any of the posts’ authors (writers) and publishers, including their affiliates and assigns. National, state, and local laws vary regarding the use and application of many of the products and treatments discussed in this website-blog. As suggested, the reader assumes the risk of any and all injuries. The Institute’s posts and reports raise many issues that are subject to change as new data emerge. None of our suggested products and protocol regimens can guarantee health benefits. Some of the links provided are for convenience, they do not constitute a formal endorsement. The publisher may not have performed independent verification of the external data contained herein, as a consequence thereto, the Institute, its founder, agents and affiliates expressly disclaim responsibility for any error in the literature. For additional details about privacy policy & terms of use, please see the Institute’s legal link, (under the Home-page’s “about” link).

 

Leave a Reply

Your email address will not be published. Required fields are marked *

*

You may use these HTML tags and attributes: <a href="" title=""> <abbr title=""> <acronym title=""> <b> <blockquote cite=""> <cite> <code> <del datetime=""> <em> <i> <q cite=""> <s> <strike> <strong>

Translate »
error: Content is protected !!