Ginger compound is effective and safe against cancer stem cells

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A new study published in PLoS confirms the anti-stem cell punch deriving from a pungent component within ginger known as 6-shogaol. The experiment targeted breast cancer cells.
As the study’s authors corroborate, cancer stem cells (CSCs) pose a serious obstacle to cancer therapy as they can be responsible for poor prognosis and tumour relapse. In this study, the authors have investigated inhibitory activity of the ginger-derived compound 6-shogaol against breast cancer cells both in monolayer and in cancer-stem cell-like spheroid culture. The spheroids were generated from adherent breast cancer cells. 6-shogaol was effective in killing both breast cancer monolayer cells and spheroids at doses that were not toxic to noncancerous cells. The percentages of CD44+CD24-/low cells and the secondary sphere content were reduced drastically upon treatment with 6-shogaol confirming its action on CSCs. Treatment with 6-shogaol caused cytoplasmic vacuole formation and cleavage of microtubule associated protein Light Chain3 (LC3) in both monolayer and spheroid culture indicating that it induced autophagy. Kinetic analysis of the LC3 expression and a combination treatment with chloroquine revealed that the autophagic flux instigated cell death in 6-shogaol treated breast cancer cells in contrast to the autophagy inhibitor chloroquine. Furthermore, 6-shogaol-induced cell death got suppressed in the presence of chloroquine and a very low level of apoptosis was exhibited even after prolonged treatment of the compound, suggesting that autophagy is the major mode of cell death induced by 6-shogaol in breast cancer cells. 6-shogaol reduced the expression levels of Cleaved Notch1 and its target proteins Hes1 and Cyclin D1 in spheroids, and the reduction was further pronounced in the presence of a γ-secretase inhibitor. Secondary sphere formation in the presence of the inhibitor was also further reduced by 6-shogaol. Together, these results indicate that the inhibitory action of 6-shogaol on spheroid growth and sustainability is conferred through γ-secretase mediated down-regulation of Notch signaling.  Conclusion: The efficacy of 6-shogaol in monolayer and cancer stem cell-like spheroids has been demonstrated for breast cancer.

ginger has been found in pre-clinical research to contain a compound up to 10,000 times more effective than the chemotherapy drug Taxol at killing breast cancer stem cells

 

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SOURCE: 6-Shogaol Inhibits Breast Cancer Cells and Stem Cell-Like Spheroids by Modulation of Notch Signaling Pathway and Induction of Autophagic Cell Death By Anasuya Ray, Smreti Vasudevan, Suparna Sengupta Published: September 10, 2015

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Advanced Cancer Research Institute’s Founder, Christian Joubert has worked as a professor of public law and holistic medicine for most of his adult life, in association with his activities in organic agriculture. Christian’s has attained expertise in many fields, thanks to which he can help as many people as possible, including, but not limited to biogerontology, (ie, extending healthy human life-spans to over 120 years old), holistic oncology, traditional naturopathy (or what is also called “natural medicine”, coming from Hippocratic Tradition), French medicine, (classified by the W.H.O. as the number one out of in 191 countries, Cf. the link “about”), comparative public policy and international public law. Trained in conventional medicine, acupuncture, naturopathy, oenology, the social sciences and law in France, Christian has campaigned and litigated in Courts of law for multiple years in order to replace outdated medical standards of care with evidence-based holistic standards. His originality is based on being able to examine medicine and the health sciences with legal & forensic eyes, thanks to which he can distinguish the relevant data from the irrelevant. In addition, his broad knowledge relative to the health sciences, organic farming, ecology, eco-development, oenology (wine science), sociology, political science, the law and different cultures allows him to identify those key inter-connections that too often, most family members, medical doctors, professors of medicine and public health experts are not able to see or understand. To benefit in vivo from Christian’s trans-cultural, medical, legal and inter-disciplinary training and experience, click the Consult link in the top menu bar.

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